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This results in defects in mitophagy, whereas a large number of dysfunctional mitochondria are not removed in time and excessively accumulate in neuronal cells, which induces a vicious circle-chain reaction (Wong and Holzbaur, 2014)

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Following fixation in 4% paraformaldehyde (PFA), 5 dpf Brn3c:mGFP larvae, which express membrane-bound GFP in hair cells, were rinsed twice with phosphate-buffered saline (PBS) for 10 min each and then once with dH 2 o for 20 min

Mixing the Solvent With the Peptide The process of mixing and injecting the solvent into lyophilized peptides determines how well they dissolve and maintain their biological activity